Work from the Lab

2023

Offley SR, Pfleiderer MM, Zucco A, Fraudeau A, Welsh SA, Razew M, Galej WP, Gardini A.
A combinatorial approach to uncover an additional Integrator subunit.
Cell Rep. 2023 Mar 28;42(3):112244. doi: 10.1016/j.celrep.2023.112244. Epub 2023 Mar 14. PMID: 36920904.

Welsh SA, Gardini A.
Genomic regulation of transcription and RNA processing by the multitasking Integrator complex.
Nat Rev Mol Cell Biol. 2023 Mar;24(3):204-220. doi: 10.1038/s41580-022-00534-2. Epub 2022 Sep 30. PMID: 36180603; PMCID: PMC9974566.

2021

Vervoort SJ, Welsh SA, Devlin JR, Barbieri E, Knight DA, Offley S, Bjelosevic S, Costacurta M, Todorovski I, Kearney CJ, Sandow JJ, Fan Z, Blyth B, McLeod V, Vissers JHA, Pavic K, Martin BP, Gregory G, Demosthenous E, Zethoven M, Kong IY, Hawkins ED, Hogg SJ, Kelly MJ, Newbold A, Simpson KJ, Kauko O, Harvey KF, Ohlmeyer M, Westermarck J, Gray N, Gardini A, Johnstone RW.
The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer.
Cell. 2021 Jun 10;184(12):3143-3162.e32. doi:10.1016/j.cell.2021.04.022. Epub 2021 May 17. PMID: 34004147; PMCID: PMC8567840.

Trizzino M, Zucco A, Deliard S, Wang F, Barbieri E, Veglia F, Gabrilovich D, Gardini A.
EGR1 is a gatekeeper of inflammatory enhancers in human macrophages.
Sci Adv. 2021 Jan 13;7(3):eaaz8836. doi: 10.1126/sciadv.aaz8836. PMID: 33523892; PMCID: PMC7806227.

2020

Barbieri E, Hill C, Quesnel-Vallières M, Zucco AJ, Barash Y, Gardini A.
Rapid and Scalable Profiling of Nascent RNA with fastGRO.
Cell Rep. 2020 Nov 10;33(6):108373. doi: 10.1016/j.celrep.2020.108373. PMID: 33176136; PMCID: PMC7702699.

2018

Barbieri E, Trizzino M, Welsh SA, Owens TA, Calabretta B, Carroll M, Sarma K, Gardini A.
Targeted Enhancer Activation by a Subunit of the Integrator Complex.
Mol Cell. 2018 Jul 5;71(1):103-116.e7. doi:10.1016/j.molcel.2018.05.031. Epub 2018 Jun 28. PMID: 30008316; PMCID: PMC6051407.

Trizzino M, Barbieri E, Petracovici A, Wu S, Welsh SA, Owens TA, Licciulli S, Zhang R, Gardini A.
The Tumor Suppressor ARID1A Controls Global Transcription via Pausing of RNA Polymerase II.
Cell Rep. 2018 Jun 26;23(13):3933-3945. doi:10.1016/j.celrep.2018.05.097. PMID: 29949775; PMCID: PMC6146183.

2016

Gardini A.
Global Run-On Sequencing (GRO-Seq).
Methods Mol Biol. 2017;1468:111-20. doi: 10.1007/978-1-4939-4035-6_9. PMID: 27662873; PMCID: PMC5880289.

Collaborations 

Liu Y, Li Q, Alikarami F, Barrett DR, Mahdavi L, Li H, Tang S, Khan TA, Michino M, Hill C, Song L, Yang L, Li Y, Pokharel SP, Stamford AW, Liverton N, Renzetti LM, Taylor S, Watt GF, Ladduwahetty T, Kargman S, Meinke PT, Foley MA, Shi J, Li H, Carroll M, Chen CW, Gardini A, Maillard I, Huggins DJ, Bernt KM, Wan L.
Small-Molecule Inhibition of the Acyl-Lysine Reader ENL as a Strategy against Acute Myeloid Leukemia.
Cancer Discov. 2022 Nov 2;12(11):2684-2709. doi:10.1158/2159-8290.CD-21-1307. PMID: 36053276; PMCID: PMC9627135.

Porazzi P, Petruk S, Pagliaroli L, De Dominici M, Deming D 2nd, Puccetti MV, Kushinsky S, Kumar G, Minieri V, Barbieri E, Deliard S, Grande A, Trizzino M, Gardini A, Canaani E, Palmisiano N, Porcu P, Ertel A, Fortina P, Eischen CM, Mazo A, Calabretta B.
Targeting Chemotherapy to Decondensed H3K27me3-Marked Chromatin of AML Cells Enhances Leukemia Suppression.
Cancer Res. 2022 Feb 1;82(3):458-471. doi: 10.1158/0008-5472.CAN-21-1297. Epub 2021 Dec 13. PMID:34903608; PMCID: PMC8889548.

Santoliquido BM, Frenquelli M, Contadini C, Bestetti S, Gaviraghi M, Barbieri E, De Antoni A, Albarello L, Amabile A, Gardini A, Lombardo A, Doglioni C, Provero P, Soddu S, Cittaro D, Tonon G.
Deletion of a pseudogene within a fragile site triggers the oncogenic expression of the mitotic CCSER1 gene.
Life Sci Alliance. 2021 Jun 29;4(8):e202101019. doi: 10.26508/lsa.202101019. PMID:34187875; PMCID: PMC8321653.

Wu S, Fatkhutdinov N, Rosin L, Luppino JM, Iwasaki O, Tanizawa H, Tang HY, Kossenkov AV, Gardini A, Noma KI, Speicher DW, Joyce EF, Zhang R.
ARID1A spatially partitions interphase chromosomes.
Sci Adv. 2019 May 22;5(5):eaaw5294. doi: 10.1126/sciadv.aaw5294. PMID: 31131328; PMCID: PMC6531001.

Carrer A, Trefely S, Zhao S, Campbell SL, Norgard RJ, Schultz KC, Sidoli S, Parris JLD, Affronti HC, Sivanand S, Egolf S, Sela Y, Trizzino M, Gardini A, Garcia BA, Snyder NW, Stanger BZ, Wellen KE.
Acetyl-CoA Metabolism Supports Multistep Pancreatic Tumorigenesis.
Cancer Discov. 2019 Mar;9(3):416-435. doi:10.1158/2159-8290.CD-18-0567. Epub 2019 Jan 9. PMID: 30626590; PMCID:PMC6643997.

Wu S, Fatkhutdinov N, Fukumoto T, Bitler BG, Park PH, Kossenkov AV, Trizzino M, Tang HY, Zhang L, Gardini A, Speicher DW, Zhang R.
SWI/SNF catalytic subunits' switch drives resistance to EZH2 inhibitors in ARID1A-mutated cells.
Nat Commun. 2018 Oct 8;9(1):4116. doi: 10.1038/s41467-018-06656-6. PMID:30297712; PMCID: PMC6175882.

Yokoyama Y, Zhu H, Lee JH, Kossenkov AV, Wu SY, Wickramasinghe JM, Yin X, Palozola KC, Gardini A, Showe LC, Zaret KS, Liu Q, Speicher D, Conejo-Garcia JR, Bradner JE, Zhang Z, Sood AK, Ordog T, Bitler BG, Zhang R.
BET Inhibitors Suppress ALDH Activity by Targeting ALDH1A1 Super-Enhancer in Ovarian Cancer.
Cancer Res. 2016 Nov 1;76(21):6320-6330. doi: 10.1158/0008-5472.CAN-16-0854. PMID: 27803105; PMCID: PMC5117661.

Postdoctoral work (Gardini)

Lai F, Gardini A, Zhang A, Shiekhattar R.
Integrator mediates the biogenesis of enhancer RNAs.
Nature. 2015 Sep 17;525(7569):399-403. doi:10.1038/nature14906. Epub 2015 Aug 26. PMID: 26308897; PMCID: PMC4718573.

Chen FX, Woodfin AR, Gardini A, Rickels RA, Marshall SA, Smith ER, Shiekhattar R, Shilatifard A.
PAF1, a Molecular Regulator of Promoter-Proximal Pausing by RNA Polymerase II.
Cell. 2015 Aug 27;162(5):1003-15. doi:10.1016/j.cell.2015.07.042. Epub 2015 Aug 13. PMID: 26279188; PMCID: PMC4679144.

Gardini A, Shiekhattar R.
The many faces of long noncoding RNAs.
FEBS J. 2015 May;282(9):1647-57. doi: 10.1111/febs.13101. Epub 2014 Nov 7. PMID: 25303371; PMCID: PMC4520312.

Gardini A, Baillat D, Cesaroni M, Hu D, Marinis JM, Wagner EJ, Lazar MA, Shilatifard A, Shiekhattar R.
Integrator regulates transcriptional initiation and pause release following activation.
Mol Cell. 2014 Oct 2;56(1):128-139. doi:10.1016/j.molcel.2014.08.004. Epub 2014 Sep 4. PMID: 25201415; PMCID: PMC4292851.

Gardini A, Baillat D, Cesaroni M, Shiekhattar R.
Genome-wide analysis reveals a role for BRCA1 and PALB2 in transcriptional co-activation.
EMBO J. 2014 Apr 16;33(8):890-905. doi: 10.1002/embj.201385567. Epub 2014 Mar 3. PMID: 24591564; PMCID: PMC4194113.

Baillat D, Gardini A, Cesaroni M, Shiekhattar R.
Requirement for SNAPC1 in transcriptional responsiveness to diverse extracellular signals.
Mol Cell Biol. 2012 Nov;32(22):4642-50. doi: 10.1128/MCB.00906-12. Epub 2012 Sep 10. PMID: 22966203; PMCID: PMC3486178.

Ørom UA, Derrien T, Beringer M, Gumireddy K, Gardini A, Bussotti G, Lai F, Zytnicki M, Notredame C, Huang Q, Guigo R, Shiekhattar R.
Long noncoding RNAs with enhancer-like function in human cells.
Cell. 2010 Oct 1;143(1):46-58. doi:10.1016/j.cell.2010.09.001. PMID: 20887892; PMCID: PMC4108080.