The Gardini Lab works to understand how mammalian genomes are expressed during cell fate specification, cellular homeostasis and tumorigenesis. 

Regulating mammalian transcription is a multi-layered process that revolves around the activity of the basal transcriptional machinery (RNAPII and associated complexes). Within the nucleus, RNAPII loading and processivity is modulated by nucleosome spacing (accessibility), histone modifications, phosphorylation switches, noncoding cis-regulatory elements and chromatin architecture. We integrate omics, genetics and biochemistry approaches to gain a holistic view of gene regulatory mechanisms.